Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Causes of papillomas on fish living in chlorinated sewage effluent /
Author Grizzle, John M.
Other Authors
Author Title of a Work
Melius, Paul.
Publisher U.S. Environmental Protection Agency, Environmental Research Laboratory ; Center for Environmental Research Information [distributor],
Year Published 1983
Report Number EPA/600-S3-82-087
OCLC Number 09816676
Subjects Fish populations--Alabama. ; Fishes--Effect of water pollution on. ; Sewage--Purification--Chlorination. ; Fish populations--Alabama--Tuskegee.
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD ARCHIVE EPA 600-S3-82-087 In Binder Headquarters Library/Washington,DC 04/12/2018
EJBD  EPA 600-S3-82-087 In Binder Headquarters Library/Washington,DC 11/14/2018
Collation 2 pages ; 28 cm
Caption title. At head of title: Project summary. "Feb. 1983." Includes bibliographical references. "EPA/600-S3-82-087."
Contents Notes
This research was initiated to determine the cause of oral papillomas on black bullheads (Ictalurus melas) from the final oxidation pond of theTuskegee, Alabama, sewage treatment plant. The water in this pond was chlorinated effluent from the sewage treatment plant. Ames-test mutagenicity of a pond-water concentrate indicated the presence of a chemical carcinogen in the pond water. However, water and sediment analysis did not identify substances suspected of causing the tumors. Cytoplasmic inclusion bodies were present in some papilloma cells. but attempts to find virions in the tumor via electron microscopy and to transmit the papillomas by means of injection of cell-free tumor homogenate into black bullheads were not successful. Juvenile black bullheads, yellow bullheads (Ictalurus natalis), and channel catfish (Ictalurus punctatus) were confined to cages in the oxidation pond and in a control pond. Most caged black bullheads in the oxidation pond developed focal, oral hyperplasia and stomatitis in the same mouth locations occupied by papillomas in wild black bullheads from this pond. These hyperplastic lesions healed in most fish during additional exposure. Mucosal hyperplasia also occurred, but at a much lower incidence, in other test species and in all control species. A similar incidence of hyperplastic lesions in black bullheads in floating and sunken cages indicated that contact with the sediment or ingestion of benthic food organisms did not affect pathogenesis of the lesion.