Record Display for the EPA National Library Catalog
RECORD NUMBER: 3 OF 4
|OLS Field Name||OLS Field Data|
|Main Title||Method 1600: Enterococci in Water by Membrane Filtration Using Membrane-Enterococcus Indoxyl-Beta-D-Glucoside Agar (mEI), July 2006.|
|CORP Author||Environmental Protection Agency, Washington, DC. Office of Water.|
|Additional Subjects||Enterococci ; Water ; Membrane filters ; Filtration ; Fluid filters ; Membranes ; Procedures ; Detection ; Enumeration ; Water quality ; Fresh water ; Marine water ; Estuarine environments ; Bacteria ; Waste water ; Potable water ; Drinking water ; Shellfish ; membrane-Enterococcus Indoxyl-Beta-D-Glucoside Agar(mEI)|
The MF method provides a direct count of bacteria in water based on the development of colonies on the surface of the membrane filter (Reference 18.5). A water sample is filtered through the membrane which retains the bacteria. Following filtration, the membrane containing the bacterial cells is placed on a selective medium, mEI agar, and incubated for 24 h at 41DGC. All colonies (regardless of color) with a blue halo are recorded as enterococci colonies. Magnification and a small fluorescent lamp are used for counting to give maximum visibility of colonies.