Record Display for the EPA National Library Catalog

RECORD NUMBER: 21 OF 395

OLS Field Name OLS Field Data
Main Title Application of DNA-DNA Colony Hybridization to the Detection of Catabolic Genotypes in Environmental Samples.
Author Sayler, G. S. ; Shields, M. S. ; Tedford, E. T. ; Breen, A. ; Hooper, S. W. ;
CORP Author Tennessee Univ., Knoxville.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Year Published 1985
Report Number EPA/600/J-85/116;
Stock Number PB86-101839
Additional Subjects Bacteria ; Toluene ; Naphthalene ; Aromatic hydrocarbons ; DNA ; Hybridization ; Chemical attack(Degradation) ; Environmental microbiology ; Molecular cloning ; Environmental pollutants ; Plasmids
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB86-101839 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 10p
Abstract
The application of preexisting DNA hybridization techniques was investigated for potential in determining populations of specific gene sequences in environmental samples. Cross-hybridizations among two degradative plasmids, TOL and NAH, and two cloning vehicles, pLAFRI and RSF1010, were determined. The detection limits for the TOL plasmid against a nonhomologous plasmid-bearing bacterial background was ascertained.