Abstract |
Survival of human bronchial epithelial cells after administration of four chemical carcinogens was measured in a clonal assay. Human bronchial epithelial cells were obtained from outgrowths of explanted tissue pieces. Serum-free medium was used for both explant culture and clonal growth. The clonal assay could be performed on three substrata: plastic dishes alone, protein-coated dishes, and inactivated Swiss 3T3 cells. Several other cell lines supported clonal growth of the human cells. Little variation among individuals in cytotoxicity of N-methyl-N'-nitro-N-nitrosoguanidine and K2CrO4, a possible human lung carcinogen, was observed, but in the case of benzo(a)pyrene and 7,12-dimethylbenz(a)anthracene, large variations in survival were found between cultures derived from different individuals. (Copyright (c) IRL Press Ltd.) |