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RECORD NUMBER: 12 OF 27

OLS Field Name OLS Field Data
Main Title Culture of animal cells : a manual of basic technique /
Author Freshney, R. Ian.
Publisher Wiley,
Year Published 2000
OCLC Number 41266026
ISBN 0471348899; 9780471348894
Subjects Tissue culture--Laboratory manuals. ; Cell culture--Laboratory manuals. ; Celkweek. ; Dieren. ; Tissus (histologie)--Cultures et milieux de culture--Manuels de laboratoire. ; Cellules--Cultures et milieux de culture--Manuels de laboratoire. ; Tissus (histologie)--Manuels de laboratoire. ; Biotechnologie. ; Cell Culture Techniques.
Internet Access
Description Access URL
Contributor biographical information http://catdir.loc.gov/catdir/enhancements/fy0607/99023536-b.html
Publisher description http://catdir.loc.gov/catdir/description/wiley032/99023536.html
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
EKCM  QH585.2.F74 2000 CEMM/GEMMD Library/Gulf Breeze,FL 12/15/2014
Edition 4th ed.
Collation xxvi, 577 pages : illustrations (some color) ; 29 cm
Notes
Includes bibliographical references (pages 523-561) and index.
Contents Notes
"The Fourth Edition of Culture of Animal Cells: A Manual of Basic Technique offers the most complete training manual of its kind on the fundamental principles and techniques of animal cell culture. Within this volume, indispensable updates reflecting the latest progress in media, specialized techniques, biotechnology, DNA transfer, and tumor culture have been made. This edition has five new chapters expanding on serum-free media, scale-up and biofermentors, molecular techniques, immortalization, and troubleshooting." "Biomedical researchers in cell biology, cytology, molecular biology, immunology, neuroscience, toxicology, and cancer biology will find Culture of Animal Cells: A Manual of Basic Technique, Fourth Edition to be an invaluable reference."--Jacket. 1. Introduction -- 2. Biology of Cultured Cells -- 3. Design and Layout -- 4. Equipment -- 5. Aseptic Technique -- 6. Safety -- 7. Culture Vessels -- 8. Media -- 9. Serum-Free Media -- 10. Preparation and Sterilization -- 11. Primary Culture -- 12. Cell Lines -- 13. Cloning and Selection -- 14. Cell Separation -- 15. Characterization -- 16. Differentiation -- 17. Transformation -- 18. Contamination -- 19. Cryopreservation -- 20. Quantitation -- 21. Cytotoxicity -- 22. Specialized Cells -- 23. Tumor Cells -- 24. Organotypic Culture -- 25. Scale-Up -- 26. Specialized Techniques -- 27. Molecular Techniques -- 28. Problem Solving.