Main Title |
Development of an Intact Hepatocyte Activation System for Routine Use with the Mouse Lymphoma Assay. |
Author |
Brock, K. H. ;
Moore, M. M. ;
Oglesby, L. A. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC. |
Year Published |
1987 |
Report Number |
EPA/600/J-87/194; |
Stock Number |
PB88-165998 |
Additional Subjects |
Hepatocytes ;
Lymphoma ;
In viro analysis ;
Mice ;
Rats ;
Cell cultures ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB88-165998 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
13p |
Abstract |
The authors have developed a method for cocultivating primary rat hepatocytes with L5178Y/TK+/- 3.7.2C mouse lymphoma cells. The system should provide a means to simulate more closely in vivo metabolism compared to metabolism by liver homogenates, while still being useful for routine screening. Hepatocytes were isolated from 200-250 g adult male Sprague-Dawley rats. Rapid attachment of the hepatocytes (2 h) was obtained by using fibronectin-coated 25-cm2 tissue culture flasks. Several factors proved important in obtaining optimal cocultivated mouse lymphoma cell growth. These included maintaining the cocultivated cultures on a rocker platform instead of stationary and either (1) reducing the cocultivation time from 16 to 4 h or (2) utilizing less than 1 x 10 to the 6th power hepatocytes per flask for 16-h exposures. CP, DMN, DMBA, and B(a)P were used to demonstrate the capability of the system to detect mutagens that require metabolic activation. |