Record Display for the EPA National Library Catalog
RECORD NUMBER: 918 OF 1234Main Title | Protein Folding Kinetics Biophysical Methods / [electronic resource] : | ||||
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Type | EBOOK | ||||
Author | Nölting, Bengt. | ||||
Publisher | Springer Berlin Heidelberg, | ||||
Year Published | 2006 | ||||
Call Number | QD415-436 | ||||
ISBN | 9783540272786 | ||||
Subjects | Life sciences ; Biotechnology ; Food science ; Biochemistry ; Biomedical engineering | ||||
Internet Access |
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Edition | Second Edition. | ||||
Collation | XIV, 222 p. 170 illus., 12 in color. online resource. | ||||
Notes | Due to license restrictions, this resource is available to EPA employees and authorized contractors only |
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Contents Notes | Structures of proteins -- Physical interactions that determine the properties of proteins -- Calculation of the kinetic rate constants -- High kinetic resolution of protein folding events -- Kinetic methods for slow reactions -- Resolution of protein structures in solution -- High structural resolution of transient protein conformations -- Experimental problems of the kinetic and structural resolution of reactions that involve proteins -- The folding pathway of a protein (barstar) at the resolution of individual residues from microseconds to seconds -- Highly resolved folding pathways and mechanisms of six proteins -- Structural determinants of the rate of protein folding -- Evolutionary computer programming of protein structure and folding -- Conclusions. The book gives a deep insight into the principles and concepts of the kinetic and structural resolution of fast chemical and biophysical reactions of proteins with emphasis on protein-folding reactions. The study of fast protein-folding reactions and the understanding of the folding paradox have significantly advanced due to the recent development of new biophysical methods which allow not only kinetic resolution in the sub-millisecond time scale but also structural resolution with unprecedented precision. Pathways and structures of early and late folding events and the transition state structures of fast- and ultrafast-folding proteins can now be studied in far more detail. Important techniques include biophysical, chemical, molecular biological and mathematical methods, in particular protein engineering, Phi-value analysis, time-resolved circular dichroism, optical triggers and pulsed infrared LASER methods, pressure and temperature jump, ultrafast mixing, stopped flow and quenched flow, dielectric relaxation and electric-field-jump, acoustic relaxation, fluorescence- and isotope-labeling, H/D exchange methods, NMR line broadening and stopped-flow NMR, transition state theory, solutions of rate equations, and evolutionary computer programming. Protein Folding Kinetics - Biophysical Methods is written for students and researchers in biochemistry, biophysics, and related fields. Special features in the second edition: - Includes detailed information and 12 color figures on the high resolution of folding transition states. - Discusses structural determinants of the rate of protein folding on a timescale from microseconds to seconds. - Provides information on self-evolving computer programs for protein-folding simulations and protein-structure predictions. |