Main Title |
Biological and chemical methodologies for assessing human exposure to airborne mutagens indoors. {microfiche} |
Author |
Matsushita, H. ;
Goto, S. ;
Endo, O. ;
Koyano, M. ;
Tanabe., K.
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;National Inst. of Public Health, Tokyo (Japan). ;Azabu Univ., Sagamihara (Japan). School of Veterinary Medicine. |
Publisher |
US Environmental Protection Agency, Health Affects Research Laboratory |
Year Published |
1990 |
Report Number |
EPA/600/D-90/162 |
Stock Number |
PB91-133025 |
Additional Subjects |
Mutagens ;
Carcinogens ;
Exposure ;
Aromatic polycyclic hydrocarbons ;
Salmonella typhimurium ;
Smoke ;
Personnel monitoring ;
Foreign technology ;
Indoor air pollution ;
Mutagenicity tests ;
Air pollution effects(Humans) ;
Benzo(a)pyrene ;
Fluorimetry ;
High pressure liquid chromatography
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-133025 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
8 p.: ill. ; 28 cm. |
Abstract |
Two highly sensitive methods have been developed and applied to the monitoring of personal exposure to airborne mutagens and carcinogenic polycyclic aromatic hydrocarbons (PAH). The automatic method for PAH analysis consists of ultrasonic extraction and multicolumn HPLC/computer controlled spectrofluorometric detection, by which 7 PAHs in indoor and/or personal particulate samples were analyzed. Concentration of PAHs, e.g., benzo(a)pyrene, indoors increased remarkably by smoking in a poorly ventilated room. The second method, an ultra microsuspension forward mutation assay using Salmonella typhimurium TM677, is about 100 times more sensitive than the ordinary Ames mutation bioassay. Mutagenicity of airborne particles collected by personal sampling was determined by this method. Personal exposures to ETS were greatest for smokers, followed by passive smokers and lowest for nonsmokers suspension. |