Main Title |
Quantitative Image Cytometry of Hepatocytes Expressing Gamma-Glutamyl Transpeptidase and Glutathione S-Transferase in Diethylnitrosamine-Initiated Rats Treated with Phenobarbitol and/or Phthalate Esters. |
Author |
Carter, J. H. ;
Richmond, R. E. ;
Carter, H. W. ;
Potter, C. L. ;
Daniel, F. B. ;
|
CORP Author |
Health Effects Research Lab., Cincinnati, OH. Genetic Toxicology Div. ;Wood Hudson Cancer Research Lab., Newport, KY. ;Northern Kentucky Univ., Highland Heights. Dept. of Biological Sciences. |
Publisher |
c1992 |
Year Published |
1992 |
Report Number |
EPA/600/J-92/317; |
Stock Number |
PB92-227586 |
Additional Subjects |
Liver ;
Gamma-glutamyltransferase ;
Glutathione transferases ;
Diethylnitrosamine ;
Phenobarbital ;
Cells(Biology) ;
Rats ;
Immunohistochemistry ;
Carcinogens ;
Pathology ;
Reprints ;
Phthalate esters ;
Image cytometry
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB92-227586 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
13p |
Abstract |
Image cytometry was used to quantify the volume of liver tissue expressing two widely accepted biochemical markers of neoplasia, gammaglutamyl transpeptidase (GGT) and the placental isozyme of glutathione s-transferase (GST-P). Rats were treated with the hepatocarcinogen, diethylnitrosamine (DENA) alone or combined with phenobarbital (BP). Other groups of rats received either diethylhexylphthalate (DEHP) or di-n-octylphthalate (DOP) in their diets for 26 weeks. GGT expression was detected diffusely throughout the liver parenchyma in several treatment groups so that any enhanced expression in AF and N was not apparent. GST-P was detected only in AF and N. GST-P may represent a second genetic alteration as GST-P(+) AF and N also expressed GGT but not the reverse. By using the stereologic Principle of Delesse (area%=volume%) and the known liver weight, the grams of tissue expressing either marker was quantified. The peroxisome proliferator, DEHP, inhibited expression of GGT or GST-P in livers of either DENA treated or DENA+PB treated rats. With GST-P, the reduction was correlated to a reduced number of AF and N. |