Main Title |
Simplified Micro Perchlorination Method for Polychlorinated Biphenyls in Biological Samples. |
Author |
Crist, Howard L. ;
Moseman., Robert F. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, N.C. Environmental Toxicology Div. |
Year Published |
1977 |
Report Number |
EPA/600/J-77/102; |
Stock Number |
PB-278 379 |
Additional Subjects |
Chemical analysis ;
Tissues(Biology) ;
Milk ;
Humans ;
Quantitative analysis ;
Contaminants ;
Toxicology ;
Environments ;
Reprints ;
Analytical methods ;
Biphenyl/chloro ;
Adipose tissue ;
Microanalysis ;
Analytical methods ;
Toxic substances
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB-278 379 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
7p |
Abstract |
A simplified methodology is presented for the micro determination of polychlorinated biphenyls (PCBs) in biological samples, by conversion to the decachlorobiphenyl (DCB) derivative. Beef adipose tissue and human milk extracts were fortified with PCB standards at 0.1-5.0 ppm, and perchlorinated with antimony pentachloride (SbCl5). Several Aroclors representing various degrees of chlorine content were investigated to assess the efficiency of conversion to DCB. Samples were cleaned up on a Florisil mini column and the PCBs were quantitated by electron capture GLC. Several chlorinated pesticides which were subjected to the perchlorination procedure did not interfere. As little as 0.1 ppm PCBs in 500 mg tissue extract can be recovered at 79-99%. The background DCB content of several brands of SbCl5 was determined. The levels of PCBs in human milk obtained by perchlorination technique are compared with data acquired by electron capture gas-liquid chromatography in which the individual chlorobiphenyls in the sample are measured. |