Record Display for the EPA National Library Catalog
RECORD NUMBER: 268 OF 377Main Title | Reoviruses in water pollution testing : final report / | |||||||||||
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Author | Spendlove, Rex S. ; Barnett, B. B. ; George, D. B. ; Adams, D. J. ; Ridinger, D. N. | |||||||||||
CORP Author | Utah State Univ., Logan.;Health Effects Research Lab., Research Triangle Park, NC. | |||||||||||
Publisher | U.S. Environmental Protection Agency, | |||||||||||
Year Published | 1984 | |||||||||||
Report Number | EPA/600/1-84/022; EPA-R-806527 | |||||||||||
Stock Number | PB85-125847 | |||||||||||
Subjects | Reoviruses ; Sewage | |||||||||||
Additional Subjects | Reoviruses ; Water pollution ; Tests ; Sewage ; Enzymes ; Recovery ; Bioassay ; Tolerances(Physiology) ; Protamines ; Precipitation(Chemistry) ; Evaluation ; Abundance ; Safety ; Rotoviruses | |||||||||||
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Collation | 59 pages ; 28 cm | |||||||||||
Abstract | Twelve cell lines were tested to determine their sensitivity to reoviruses of three serotypes that had been isolated from sewage. Madin-Darby bovine kidney (MDBK) cells were the most susceptible. Sewage-isolated, protamine-precipitated reoviruses were also used in conjunction with MDBK cells in a comparative evaluation of immunofluorescent cell count (ICC) and plaque assay procedures. Immunofluorescence assay is more sensitive and more rapid than plaque assay procedure for quantifying reoviruses recovered from sewage. A procedure for protamine sulfate precipitation of reoviruses and rotaviruses was developed. Some reoviruses recovered from sewage were inactivated by enzyme treatment. This report describes a study to evaluate reoviruses for their potential usefulness in water quality testing. |
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Notes | Caption title. "Final Report." "Nov. 1984." "EPA-600/1-84-022." Microfiche. |