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Main Title Acid-fast bacteria and yeasts as indicators of disinfection efficiency /
Author Engelbrecht, Richard S., ; Haas, Charles N. ; Shular, Jeffrey A. ; Dunn, David L. ; Roy, Dipak
Other Authors
Author Title of a Work
Haas, Charles N.,
Shular, Jeffrey A,
Dunn, David L.,
Dipak, Roy,
Lalchandani, Ajit,
Severin, Blaine F.,
Farooq, Shukat,
Engelbrecht, Richard S.
CORP Author Illinois Univ. at Urbana-Champaign. Dept. of Civil Engineering.;Municipal Environmental Research Lab., Cincinnati, OH.
Publisher Environmental Protection Agency, Office of Research and Development, Municipal Environmental Research Laboratory ; For sale by the National Technical Information Service,
Year Published 1979
Report Number EPA-600/2-79-091
Stock Number PB80-124795
OCLC Number 06535316
Subjects Yeast ; Ozonization ; Chlorination ; Drinking water
Additional Subjects Bacteria ; Yeasts ; Disinfection ; Potable water ; Indicator species ; Efficiency ; Microorganism control(Water) ; Chlorination ; Ozonization ; Water treatment ; Coliform bacteria ; Evaluation ; Bioindicators ; Drinking water ; Candida parapsilosis ; Mycobacterium fortuitum
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EHAM  TD747.A34 Region 1 Library/Boston,MA 04/29/2016
EJBD  EPA 600-2-79-091 Headquarters Library/Washington,DC 09/15/2014
EKBD  EPA-600/2-79-091 Research Triangle Park Library/RTP, NC 09/07/2001
ELBD ARCHIVE EPA 600-2-79-091 Received from HQ AWBERC Library/Cincinnati,OH 10/04/2023 DISPERSAL
ESAD  EPA 600-2-79-091 Region 10 Library/Seattle,WA 03/23/2010
NTIS  PB80-124795 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation xii, 143 pages : illustrations ; 28 cm.
Since the coliform group of organisms is considered to be less resistant to chlorine than some bacterial and viral pathogens, the utility of both yeast and acid-fast oganisms as potntial indicators of disinfection efficiency was evaluated. In most laboratory studies these two groups of organisms were represented by Candida parapsilosis and Mycobacterium fortuitum, respectively. The relative resistance of the rest organisms to free chlorine was: acid fast greater than yeast greater than coliforms. The increased chlorine resistance of these organisms appeared to be the result of the thickness and impermeability of the cell wall. It was concluded that the primary mode of action of chlorine in disinfection was disruption of the cell membrane with a resultant change in cell permeability and physical damage to the cell DNA. Resistance to ozonation was also studied. Variations in pH between 5 and 10 did not significantly affect organism survival of either yearor acid-fast organisms using constant ozone residuals, while increasing temperatures increased the inactivation of both organisms. Large volume sampling and enumeration techniques were developed for the yeasts and acid-fast organisms using membrane filtration which enabled the enumeration of these organisms at the relatively low densities found in finished drinking water.
EPA-600/2-79-091. Includes bibliographical references.