Record Display for the EPA National Library Catalog


Main Title Simplified Feeding Techniques for Culturing and Toxicity Testing with 'Ceriodaphnia'.
Author Keen, R. E. ; Spence, J. A. ;
CORP Author Michigan Technological Univ., Houghton. Dept. of Biological Sciences.;Environmental Research Lab.-Duluth, MN.
Year Published 1988
Report Number EPA-R-813070; EPA/600/3-88/033;
Stock Number PB88-246079
Additional Subjects Toxicity ; Daphnia ; Diets ; Growth ; Culture media ; Tests ; Algae ; Frozen foods ; Mixtures ; Lipids ; Fatty acids ; Vitamins ; Bacteria ; Survival ; Yeast ; Reproduction(Biology) ; Food preparation ; Ceriodaphnia dubia ; Freeze-dried food
Library Call Number Additional Info Location Last
NTIS  PB88-246079 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 56p
Food used for Ceriodaphnia dubia in culture and toxicity testing should be uniform, adequate, and easily prepared. Using as a control the current standard food, YCTF, the authors tested a variety of foods made with algal species singly and in combination, prepared with several methods of freezing and freeze-drying. The measure of food usefulness was a combination of survival and reproduction over a 7-day period. Foods based on single algal species were not as useful as those with a mix of species, and the easily used freeze-dried foods were better than the frozen preparations. Several commercial plankton foods, including yeast, algae, microcapsules and microparticulates, failed to support Ceriodaphnia adequately. In a separate series of experiments, the authors tried freeze-dried YCTF mixtures as a food. The preparation proved consistently capable of supporting good growth and reproduction of Ceriodaphnia.