Flow cytometry : an introduction / Alice L. Givan -- Multiparameter flow cytometry of bacteria / Howard M. Shapiro and Gerhard Nebe-von-Caron -- Multiparameter data acquisition and analysis of leukocytes / Carleton C. Stewart and Sigrid J. Stewart -- Flow cytometric analysis of kinase signaling cascades / Omar D. Perez, Peter O. Krutzik, and Garry P. Nolan -- Cytokine flow cytometry / Holden T. Maecker -- Use of cell-tracking dyes to determine the proliferation precursor frequencies of antigen-specific t cells / Alice L. Givan [and others] -- Assessment of lymphocyte-mediated cytotoxicity using flow cytometry / Luzheng Liu [and others] -- Multiparametric analysis of apoptosis by flow and image cytometry / William G. Telford, Akira Komoriya, and Beverly Z. Packard -- Detection and enrichment of hematopoietic stem cells by side population phenotype / Shannon S. Eaker [and others] -- Hematopoietic stem cell characterization by hoechst 33342 and rhodamine 123 staining / Ivan Bertoncello and Brenda Williams -- Phenotypic and functional analyses of human CD34neg hematopoietic precursors from mobilized peripheral blood / Douglas C. Dooley and Barbara K. Oppenlander -- Multiparameter flow cytometry of fluorescent protein reporters / Teresa S. Hawley [and others]. Analysis of fluorescent protein expressing cells by flow cytometry / Steven C. Pruitt, Lawrence M. Mielnicki, and Carleton C. Stewart -- Integrative flow cytometric and microarray approaches for use in transcriptional profiling / David W. Galbraith, Rangasamy Elumalai, and Fang Cheng Gong -- Flow cytometric analysis of fluorescence resonance energy transfer : a tool for high-throughput screening of molecular interactions in living cells / Francis Ka-Ming Chan and Kevin L. Holmes -- Design of a fluorescence-activated cell sorting based mammalian protein/protein interaction trap / Sam Lievens [and others] -- Flow cytometric screening of yeast surface display libraries / Michael Feldhaus and Robert Siegel -- Fluorescence resonance energy transfer based hiv-1 virion fusion assay -- Marielle cavrois [and others] -- Cell cycle analysis of asynchronous populations / Michael G. Ormerod -- Solid tumor dna content analysis / Adel K. El-Naggar and Philippe Vielh -- Concurrent flow cytometric analysis of DNA and RNA / Adel K. El-Naggar -- Telomere length measurement by fluorescence in situ hybridiziation and flow cytometry / Veena Kapoor and William G. Telford -- Small lasers in flow cytometry / William G. Telford -- Viable infectious cell sorting in a BSL-3 facility / Stephen P. Perfetto [and others]. This thoroughly revised and updated edition of a widely used practical guide to flow cytometry describes in step-by-step detail an array of time proven and cutting-edge techniques much needed in today's advanced laboratories. These readily reproducible methods deploy emerging flow cytometry technologies in many new applications, especially in the field of stem cells, functional genomics and proteomics, and microbiology. Here, the aspiring investigator will find methods for the characterization of stem/progenitor cells by monitoring the efflux of fluorescent dyes and the elucidation of signal transduction pathways using phospho-specific antibodies. There are also techniques for monitoring gene transfer and expression using fluorescent protein technology, high throughput screening for discovery of novel protein interactions, phenotypic and functional characterization of T cell subsets and precursors, and microbial flow cytometry, to highlight but some of the many useful procedu.