Record Display for the EPA National Library Catalog


Main Title Method 1605 : Aeromonas in finished by membrane filtration, September 2000-draft.
CORP Author Environmental Protection Agency, Washington, DC. Office of Water.
Publisher U.S. Environmental Protection Agency, Office of Water,
Year Published 2000
Report Number EPA/821/R-00/026
Stock Number PB2001-101413
OCLC Number 49863225
Additional Subjects Membrane filtration ; Bacteria ; Water sampling ; Drinking water ; Filtration ; Growth(Biology) ; Water filters ; Water analysis ; Pore size ; Dextrins ; Incubation ; Agar ; Fermentation ; Carbohydrates ; Acidity ; Chemical indicators ; Colors ; Cytochrome oxidase ; Trehalose ; Water pollution effects ; Surface waters ; Contamination ; Quality control ; Aeromonas ; Ampicillin ; Bromothymol blue ; Oxidase tests ; Cytochrome C
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD  EPA 821-R-00-026 c.1 Headquarters Library/Washington,DC 01/08/2014
ELBD ARCHIVE EPA 821-R-00-026 Received from HQ AWBERC Library/Cincinnati,OH 10/04/2023
ESAD  EPA 821-R-00-026 Region 10 Library/Seattle,WA 05/24/2002
NTIS  PB2001-101413 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 28 p. : ill. ; 28 cm.
The method provides a direct count of Aeromonas in water based on the development of yellow colonies on the surface of the membrane filter using a selective media for Aeromonas species. A water sample is filtered through 0.45 micrometer membrane filter. The filter is placed on ampicillin-dextrin agar and incubated at 35 C +/- 0.5 C for 22 to 26 hours. This medium uses 10-milligram/liter ampicillin to inhibit non-Aeromonas species, while allowing Aeromonas to grow. The medium uses dextrin as a fermentable carbohydrate, and bromothymol blue as an indicator of acidity produced by the fermentation of dextrin. Yellow presumptively identified colonies are counted and confirmed by testing for the presence of cytochrome c (oxidase test) and the ability to ferment trehalose. The membrane filtration procedure provides a direct count of culturable Aeromonas in water samples that is based on the bacterial growth on the surface of the membrane.
Cover title. "September 2000." "EPA 821-R-00-026."