This report contains the findings of the Public Health Service investigation and information concerning water supplies, pollution sources, and general water quality in Twelvepole Creek Basin, West Virginia as obtained in a field investigation. The Twelvepole Creek Basin is described in terms of population, geography, and industry. Sources of waste are domestic and acid mine drainage. Surface water is evaluated, and ground water is analyzed. Reservoir sites for proposed water storage are discussed with the consideration of flow augmentation to determine the most beneficial and feasible plan for the development and control of the water resources of the Basin.