||Biochemical Effects of Two Promoters of Hepatocarcinogenesis in Rats.
Kitchin, K. T. ;
Brown, J. L. ;
||Health Effects Research Lab., Research Triangle Park, NC. Experimental Biology Div.
Liver neoplasms ;
Deoxyribonucleic acids ;
Alpha amino carboxylic acids ;
Valeric acid ;
Butylated hydroxytoluene ;
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The effects of administration of two promoters of hepatocarcinogenesis on five hepatic biochemical parameters were examined in adult female rats. Two treatments of phenobarbital (100 mg/kg) 21 and 4 hours before sacrifice caused large increases in hepatic ODC activity and cytochrome P-450 content. Extending the number of phenobarbital treatments to 5 increased the size of hepatic enzyme induction and caused a minor decrease in hepatic glutathione and a small increase in serum alanine aminotransferase. Two administrations of butylated hydroxytoluene (BHT) (700 mg/kg) caused hepatic DNA damage and induction of both ODC activity and cytochrome P-450 content. Of the two compounds only BHT showed a potential for carcinogenic initiation. The biochemical parameters studied here, may form a useful biochemical system to examine a compound's potential to cause carcinogenic initiation, carcinogenic promotion and cellular toxicity.