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Main Title Aneuploidy Detection with a Short-Term Hexaploid Wheat Assay (Journal Version).
Author Redei, G. P. ; Sandhu, S. S. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Missouri Univ.-Columbia.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/204;
Stock Number PB89-144463
Additional Subjects Detection ; Wheat plants ; Mutagens ; Bioassay ; Chromosome abnormalities ; Toxicity ; X-rays ; Caffeine ; Phenylalanine ; Triazoles ; Vinblastin ; Gamma rays ; Reprints ; Aneuploidy ; Neatby's virescens ; Genetic nondisjunction ; Benzopyrenes ; Auramine ; Diethylstilbestrol ; Dichlorvos ; Safrole ; Sulfacetamide
Library Call Number Additional Info Location Last
NTIS  PB89-144463 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
A novel assay for the identification of agents causing aneuploidy is described. This assay takes advantage of allohexaploid wheat in which monosomic and nullisomic cell lineages can be genetically detected. The wheat strain used is homozygous for a pair of recissive alleles (v1) which in homozygous condition interfere with normal pigmentation of the leaves at low temperature, but it is hemizygous ineffective. This locus is in the short arm of chromosome 3B near the centromere. As a consequence of nondisjunction of this chromosome, twin sectors may be detected in which the monosomic cell lineages appear green, whereas the trisomic sectors display white color on a cream-colored background at low temperature. This genetic system can also be used for the detection of deletions or duplications involving the short arm of chromosome 3B. Results show that X-rays, gamma rays, p-fluorophenyl-alanine, 3-amino-triazole, caffeine, vinblastin sulfate, benzo(a)pyrene, and auramine significantly increased aneuploidy, and diethylstilbestrol, sulfacetamide, safrole, and dichlorvos caused some increase of sectoring.