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OLS Field Name OLS Field Data

Main Title Transfection with Baculovirus DNA.

Author Burand, John P. ; Summers, Max D. ; Smith, Gale E. ;

CORP Author Texas A and M Univ., College Station. Dept. of Entomology.;Health Effects Research Lab., Research Triangle Park, NC.

Year Published 1980

Report Number EPA-R-805232-01; EPA-600/J-80-257;

Stock Number PB81-191959

Additional Subjects Viruses ; Deoxyribonucleic acid ; Molecular biology ; Microorganisms ; Cells(Biology) ; Reprints ; Autographa californica ; Rachiplusia ou ; Transfection

Holdings

Library	Call Number	Additional Info	Location	Last Modified	Checkout Status
NTIS	PB81-191959	Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.		06/23/1988

Collation 8p

Abstract

Purified DNA from the nuclear polyhedrosis viruses of Autographa californica (AcM NPV) and Rachiplusia ou (RoM NPV) were found to be infectious in TN-368 cells employing the calcium phosphate precipitation technique (F.L. Graham and A.J. van der Eb, Virology, 52, 456-467, 1973). Transfection with AcMNPV produced 3600 PFU/microgram DNA compared to 2900 PFU/microgram DNA with RoMNPV. Of eight baculovirus DNAs tested, only AcMNPV DNA and RoMNPV DNA could transfect TN-368 cells. The in vitro host range of AcMNPV DNA was determined to be the same as AcMNPV extracellular virus. AcMNPV Form 1 DNA was fourfold more infectious in TN-368 cells than Form II DNA.

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