Record Display for the EPA National Library Catalog


Main Title Colony Formation Enhancement of Rat Tracheal and Nasal Epithelial Cells by Polyacetate, Indole Alkaloid, and Phorbol Ester Tumor Promoters.
Author Mass, M. J. ; Lasley, J. A. ; Marr, C. M. ; Arnold, J. T. ; Steele, V. E. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/J-87/009;
Stock Number PB87-184677
Additional Subjects Carcinogens ; Epithelium ; Trachea ; Nose(Anatomy) ; Rats ; Laboratory animals ; Neoplasms ; Reprints ; Tumor promoters ; Colony forming units ; Polyacetate ; Indole alkaloid ; Phorbol ester
Library Call Number Additional Info Location Last
NTIS  PB87-184677 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 5p
The phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA), teleocidin, and two polyacetate tumor promoters (aplysiatoxin and debromoaplysiatoxin) have been tested for their effect on colony forming efficiency (CFE) of rat tracheal and nasal turbinate epithelial cells. In rat tracheal epithelial (RTE) cells, all four compounds stimulated colony formation by up to eight-fold using picomolar concentrations of aplysiatoxin and teleocidin, whereas TPA and debromoaplysiatoxin were effective in the nanomolar range. In addition, teleocidin and the other promoters increased the number of cells in colonies by 3-5 fold resulting in larger colonies, most notably above concentrations that maximally stimulated CFE, In contrast, rat nasal epithelial cells were only marginally stimulated by these tumor promoters to form colonies. The results indicate that there is regional specificity in responses to tumor promoters and RTE cells can act as very sensitive biological indicators of the presence of these three classes of tumor promoters with diverse structure. (Copyright (c) Carcinogenesis vol. 8 no. 1 p. 179-181, 1987.)