Nitrogen, phosphorus, and sediment loads to the Great Bay Estuary are a growing concern. The Piscataqua Region Estuaries Partnership (PREP) calculates the nitrogen load from tributaries to the Great Bay Estuary for its State of the Estuaries reports. Therefore, the purpose of this study was to collect representative data on nitrogen, phosphorus, and suspended sediment concentrations in tributaries to the Great Bay Estuary in 2009. The study design followed the tributary sampling design which was implemented by the New Hampshire Department of Environmental Services between 2001 and 2007 and by the University of New Hampshire in 2008, so as to provide comparable data to the previous loading estimates.