Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Effects of Strain, Sex, Route of Administration and Partial Hepatectomy on the Induction by Chemical Carcinogens of Gamma-Glutamyltranspeptidase Foci in Rat Liver.
Author Pereira, M. A. ; Herren-Freund, S. L. ; Britt, A. L. ; Khoury, M. M. ;
CORP Author Health Effects Research Lab., Cincinnati, OH. ;Cincinnati Univ., OH. Coll. of Medicine.
Year Published 1983
Report Number EPA/600/J-83/234;
Stock Number PB84-243815
Additional Subjects Hepatectomy ; Liver ; Rats ; Stress(Physiology) ; Sex ; Surgery ; Reprints ; Carcinogens
Library Call Number Additional Info Location Last
NTIS  PB84-243815 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 11p
The incidence of gamma-glutamyltranspeptidase (GGT)-positive foci induced by 0.3 mmol/kg diethylnitrosamine (DENA) followed by promotion with 500 ppm sodium phenobarbital in drinking water and was the same in Fischer 344, Sprague-Dawley and Wistar-Lewis rats. There was no difference in the level of GGT-foci initiated by DENA, 7,12-dimethylbenz(a)-anthracene (DMBA), or 1,2-dimethylhydrazine (DMH) followed by promotion with phenobarbital with respect to sex or route of administration including gavage and intraperitoneal injection. Maximal stimulation by partial hepatectomy of DENA initiation of GGT-foci occurred when the DENA was administered 18 h after the operation. The results indicate that the optimal protocol for the rat liver foci assay consists of using partial hepatectomized rats of 1 of the 3 strains and of either sex. The test substance should be administered by either gavage or intraperitoneal injection so that maximal DNA binding coincides with the maximal rate of DNA replication resulting from partial hepatectomy.