Twenty-five stations on the Kishwaukee River and tributaries were sampled for water and sediment quality, macroinvertebrates, fish and habitat in 1983. From these data, environmental quality was summarized utilizing various indices including water quality (WQI), macroinvertebrates (MBI), fish (AIBI), and habitat (PIBI). Individual stations were evaluated for degree of support of aquatic life and classified according to the biological stream classification system used for Illinois streams. All mean index values indicated good to very good environmental conditions providing full aquatic life use at 22 of 25 stations (88%).