Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Formation of DNA Adducts in 1OT1/2 Mouse Embryo Fibroblasts Incubated with Benzo(a)pyrene or Dihydrodiol Oxide Derivatives.
Author Brown, Halina Szejnwald ; Jeffrey, Alan M. ; Weinstein, I. Bernard ;
CORP Author Columbia Univ., New York.;Health Effects Research Lab., Research Triangle Park, NC.;National Cancer Inst., Bethesda, MD.
Year Published 1979
Report Number EPA-R-805482 ;PHS-CA-13696; EPA-600/J-79-148;
Stock Number PB83-145292
Additional Subjects Deoxyribonucleic acid ; Aromatic polycyclic hydrocarbons ; Binding ; Mice ; Embryos ; Oxygen organic compounds ; Stereochemistry ; Reprints ; Dihydrodiol oxide ; Metabolites ; Cell lines ; Benzopyrenes ; Fibroblasts
Library Call Number Additional Info Location Last
NTIS  PB83-145292 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 7p
Binding of benzo(a)pyrene (BP) metabolites to DNA was studied in the mouse embryo fibroblast 10T one half cell line. The cells metabolized BP to its diastereoisomeric 7,8-dihydrodiol-9,10-oxides, (+)-7B, 8a-dihydroxy-9B, 10B-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene, which formed covalent adducts with cellular DNA. Detailed analysis by high-pressure liquid chromatography indicated that a deoxyguanosine adduct, N2-(10S-(7R, 8S, 9R-trihydroxy-7,8,9,10-tetrahydrobenzo(a)pyrene)yldeoxyguaninosine, was the predominant DNA adduct formed in cells exposed to BP. This adduct is also the major one found in human tissues exposed to BP.