||Health Effects Research Lab., Research Triangle Park, NC. ;Research Triangle Inst., Research Triangle Park, NC. ;Keio Univ., Yokohama (Japan). Dept. of Biology. ;Guru Nanak Dev Univ., Amritsar (India). Dept. of Botanical Sciences. ;Komenskeho Univ., Bratislava (Czechoslovakia). Lab. of Karyology.
A collaborative study involving laboratories in six countries was initiated under the auspices of the International Program on Chemical Safety (IPCS), the United Nations Environment Program (UNEP), the International Labor Organization (ILO) and the World Health Organization (WHO) to determine the sensitivity, efficiency and reliability of the Vicia faba root-tip meristem chromosomal aberration assay using a standardized protocol. The six laboratories that participated in this study were located in Czechoslovakia, India, Japan, Poland, Sweden and the U.S.A. All laboratories adhered to a standardized protocol for the Vicia faba chromosomal aberration assay. Four coded chemicals, azidoglycerol (AG), methyl nitrosourea (MNU), sodium azide (NaN3), and maleic hydrazide (MH) were tested with the Vicia faba chromosomal aberration assay. Of the 4 chemicals, 3 (MH, AG and MNU) were found to be clastogenic and gave a concentration-related response. However, the results for NaN3 were equivocal, which might be explained by the stability of NaN3. The conclusions from this study suggest that the Vicia faba chromosomal aberration bioassay is an efficient and reliable short-term bioassay for the rapid screening of chemicals for clastogenicity. (Copyright (c) 1994 Elsevier Science B.V.)