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Main Title Identification, isolation and characterization of the infectious hepatitis (hepatitis A) agent /
Author Hall, William T.
Other Authors
Author Title of a Work
Clarke, N. A.
Clarke, N. A. (Norman A.)
Publisher Health Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency ; National Technical Information Service [distributor],
Year Published 1977
Report Number EPA-600/1-77-049; EPA-R-804003
Stock Number PB-274 841
OCLC Number 07154474
Subjects Hepatitis A--transmission ; Hepatitis A--etiology ; Hepatovirus--analysis ; Antigens, Viral--analysis ; Hepatitis A Virus--analysis
Additional Subjects Viruses ; Infectious hepatitis virus ; Viral diseases ; Infectious hepatitis ; Serology ; Hemagglutination ; Antibodies ; Antigens ; Electron microscopy ; Ultracentrifuging ; Tissue culture ; Assaying ; Identifying ; Isolation ; Patients ; Immunity ; Prophylaxis ; Laboratory animals ; Experimental data ; Tables(Data) ; Blood donors ; Epidemiology ; Hepatitis A virus ; Hepatitis A antigen ; Radioimmunoassay ; Immunoglobulins
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD ARCHIVE EPA 600-1-77-049 Headquarters Library/Washington,DC 06/04/2012
EJBD  EPA 600-1-77-049 c.1 Headquarters Library/Washington,DC 05/19/2014
ELBD  EPA 600-1-77-049 AWBERC Library/Cincinnati,OH 06/04/1999
NTIS  PB-274 841 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation ix, 39 p. : ill. ; 28 cm.
The research program has the overall objective of combining the techniques of electron microscopy, ultracentrifugation, column chromatography, tissue culture and serology to identify, isolate and characterize the etiologic agent of infectious hepatitis, to propagate it in cell cultures and to develop in vitro immunodiagnostic assays capable of detecting the presence of the hepatitis A virus and its antibody both in the patient and the environment. Through the program, it was possible morphologically and serologically to relate this agent to those isolated from other geographically separated hepatitis A epidemics. Employing hepatitis A antigen detected by radioimmunoassay (RIA) screening and isolated from stool specimens collected from patients with both clinical and subclinical hepatitis, a radioimmunoassay and an immune adherence hemagglutination assay were developed for the detection of antibody to hepatitis A. Using these assays, the protective effect of circulating anti-HA against reinfection and the prophylactic effect of commercial immune serum globulin (ISC) containing anti-HA were established. A survey of a commercial plasma donor population showed that approximately 37% of the tested donors had anti-HA with the frequency varying directly with age. All attempts to propagate the hepatitis A virus in tissue culture have been unsuccessful.
"EPA-600/1-77-049." "This report was submitted in fulfillment of Grant No. R-804003 by Electro-Nucleonics Laboratories, Inc." Includes bibliographical references (p. 34-38).