Record Display for the EPA National Library Catalog


Main Title Bioassay of Water Pollutants with Cultured Mammalian Cells: Part II.
Author Fishe, Harold W. ;
CORP Author Rhode Island Univ., Kingston. Water Resources Center.
Year Published 1970
Report Number Di-14-01-0001-3040; OWRR-A-030-RI; 03178,; A-030-RI(1)
Stock Number PB-205 775
Additional Subjects ( Water pollution ; Quantitative analysis) ; ( Contaminants ; Bioassay) ; ( Tissue culture ; Bioassay) ; Mammals ; Cells(Biology) ; Chemical compounds ; Toxicity ; In vitro analysis ; Rotenone ; Cadmium ; Copper ; Acetic acid ; Zinc ; Acetic acid/nitrilo-tri
Library Call Number Additional Info Location Last
NTIS  PB-205 775 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 19p
The increasing problem of environmental contamination by chemicals has suggested the potential usefulness of a cell bioassay system permitting the rapid screening of the toxicological properties of chemicals under standardized conditions. Using quantitative methods of mammalian cell culture, a series of preliminary investigations were carried out on a selected group of toxic chemicals. These studies demonstrated that the toxicity of all chemicals investigated could be determined with sufficient precision and sensitivity to establish a unique relative toxicity for each chemical and to permit an analysis of the kinetics of toxic action. In this study, bovine ovary cells plated into petri dishes were exposed to varying concentrations of rotenone, cadmium, zinc, copper, chromium, and NTA continuously for 7-9 days. The relative plating efficiency was determined and titration curves were constructed. In the kinetic phase of this study, attached cells were exposed to single concentrations of the toxic chemical for varying time intervals and the relative plating efficiency followed as a function of exposure time. The log of the surviving cell fraction was plotted against exposure time giving classical survival curves.