Record Display for the EPA National Library Catalog


Main Title Simplified Radioimmunoassay for Detection of Human Rotavirus in Stools.
Author Cukor, George ; Berry, Mary Kay ; Blacklow, Neil R. ;
CORP Author Massachusetts Univ. Medical School, Worcester.;Health Effects Research Lab., Cincinnati, OH.
Year Published 1981
Report Number EPA-R-805169 ;EPA-R-806591-01-0; EPA/600/J-78/184;
Stock Number PB81-231110
Additional Subjects Diagnosis ; Humans ; Infants ; Electron microscopy ; Reprints ; Radioimmunoassay ; Rotaviruses
Library Call Number Additional Info Location Last
NTIS  PB81-231110 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
A simplified radioimmunoassay (RIA) technique was developed to facilitate the diagnosis of human rotavirus in stools of infants with diarrhea. This microtiter solid-phase RIA utilizes as a critical reagent hyperimmune serum against a tissue culture-grown simian rotavirus that is immunologically closely related to the human agent. One hundred thirteen pediatric stool samples were tested. Thirty-five specimens known to be positive for rotavirus by electron microscopy were also positive by RIA. All RIA-positive reactions (36) were shown to be specific for rotavirus by means of an antibody-blocking test. Nonspecific RIA-positive reactions were not encountered. This accurate, sensitive RIA technique is practical for use in diagnostic laboratories because critical serum reagents are prepared against culture-grown virus and not against rotavirus derived from carefully selected stools of humans or experimentally infected gnotobiotic animals.