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Main Title Relative Concentrations of Serum Neutralizing Antibody to VP3 and VP7 Proteins in Adults Infected with a Human Rotavirus (Journal Version).
Author Ward, R. L. ; Knowlton, D. R. ; Schiff, G. M. ; Hoshino, Y. ; Greenberg, H. B. ;
CORP Author James N. Gamble Inst. of Medical Research, Cincinnati, OH. ;Veterans Administration Medical Center, Palo Alto, CA. ;National Institutes of Health, Bethesda, MD.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/129;
Stock Number PB89-110621
Additional Subjects Immunization ; Capsid ; Viral proteins ; Rotaviruses ; Antibody formation ; Humans ; Neutralization tests ; Genetic recombination ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB89-110621 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Two outer capsid rotavirus proteins, VP3 and VP7, have been found to elicit neutralizing antibody production, but the immunogenicity of these proteins during human rotavirus infection has not been determined. The relative amounts of serum neutralizing antibody against the VP3 and VP7 proteins of the CJN strain of human rotavirus were, therefore, determined in adults subjects before and after infection with this virus. Reassortant strains of rotavirus that contained the CJN gene segment for only one of these two neutralization proteins were isolated and used. The geometric mean titer of serum neutralizing antibody to a reassortant virus (CJN-M) that contained VP7 of CJN and VP3 of another human rotavirus was 12.7 times less than that of antibody to CJN before infection and 20.3 times less after infection. This indicated that most neutralizing antibody was against the CP3 rather than the VP7 protein of CJN. The result was confirmed with other reassortants between CJN and animal rotavirus strains (EDIM and rhesus rotavirus). The findings suggest that CP3 is the primary immunogen that stimulates neutralizing antibody during at least some rotavirus infections of humans. (Copyright (c) 1988 American Society for Microbiology.)