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Main Title Conditions Influencing Yield and Analysis of 8-Hydroxy-2' -Deoxyguanosine in Oxidatively Damaged DNA.
Author Floyd, R. A. ; West, M. S. ; Eneff, K. L. ; Schneider, J. E. ; Wong., P. K. ;
CORP Author Oklahoma Medical Research Foundation, Oklahoma City.;Corvallis Environmental Research Lab., OR.;National Institutes of Health, Bethesda, MD.
Publisher c1990
Year Published 1990
Report Number EPA-R-814198 ;NIH-CA-42854; EPA/600/J-90/213;
Stock Number PB91-117499
Additional Subjects Temperature ; pH ; Free radicals ; Chemical analysis ; Alkaline phosphatase ; Reprints ; DNA damage ; Hydroxydeoxyguanosine ; Restriction endonucleases ; Hydroxyl radicals ; Phosphodiesterases
Library Call Number Additional Info Location Last
NTIS  PB91-117499 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
Studies have been conducted to obtain practical knowledge regarding the stability, digestion, and analytical determination of the content of 8-hydroxy-2-deoxyguanosine (8-OHdG) in oxidatively damaged DNA. Utilizing H2O2 plus UV light to form oxidatively damaged DNA, the authors found that storage of the DNA at -20C at alkaline pH caused a significant loss of 8-OHdG, whereas storage at -20C at neutral or acidic pH prevented loss of 8-OHdG. The 8-OHdG within DNA is stable at 100C for at least 15 min. Formation of 8-OHdG within DNA using UV light and H2O2 as a hydroxyl free radical-generating system yields the highest amounts when low levels of phosphate buffer are used; but the use of Tris or citrate buffers cause a lower yield of 8-OHdG because these buffers act as scavengers for the hydroxyl free radicals. Independent assessment of hydroxyl free radical flux by the use of salicylate trapping allows assessment of competitive radical reactions. (Copyright (c) 1990 Academic Press, Inc.)