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Main Title Characterization of Stress-Responsive Behavior in 'Pseudomonas aeruginosa' PAO: Isolation of Tn3-lacZYA Fusions with Novel Damage-Inducible (din) Promoters.
Author Warner-Bartnick, A. L. ; Miller, R. V. ;
CORP Author Stritch School of Medicine, Maywood, IL. ;Oklahoma State Univ., Stillwater. Dept. of Botany and Microbiology.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/215;
Stock Number PB92-195718
Additional Subjects Pseudomonas aeruginosa ; Promotor regions(Genetics) ; Repressor proteins ; Recombinant fusion proteins ; DNA damage ; Restriction mapping ; Beta-galactosidase ; Genomic library ; SOS response(Genetics) ; Kinetics ; Ultraviolet rays ; Escherichia coli ; Norfloxacin ; Plasmids ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB92-195718 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Although the pervasive soil and water microorganism Pseudomonas aeruginosa demonstrates heightened sensitivity to UV radiation, the species possesses a recA gene that, based on structural and functional properties, could mediate a DNA damage-responsive regulon similar to the SOS regulon of Escherichia coli. To determine whether P. aeruginosa encodes such stress-inducible genes, the response of P. aeruginosa to DNA-damaging agents including far-UV radiation (UVC) and the quinolone antimicrobial agent norfloxacin was investigated by monitoring the expression of fusions linking P. aeruginosa promoters to a beta-galactosidase reporter gene. These fusions were obtained by Tn3-HoHoI insertional mutagenesis of a P. aeruginosa genomic library. Eight different damage-inducible (din) gene fusions were isolated which lack homology to the P. aeruginosa recA gene. Expression of the three gene fusions studied, dinA::lacZYA, dinB::lacZYA, and dinC::lacZYA, increased following UVC and quinolone exposure but not following heat shock. Similar to E. coli SOS genes, the din genes were induced to different extents and with dissimilar kinetics following UVC irradiation. (Copyright (c) 1992, American Society for Microbiology.)