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Main Title Quantitative PCR protocols /
Other Authors
Author Title of a Work
Kochanowski, Bernd.
Reischl, Udo.
Publisher Humana Press,
Year Published 1999
OCLC Number 40698398
ISBN 0896035182; 9780896035188
Subjects Polymerase chain reaction--Laboratory manuals ; Biologie molculaire ; ADN = Acide dšoxyribonucléique ; ARN--analyse ; ADN--analyse ; Réaction de polymérisation en chaãine--Méthodes ; Polymerase kettingreactie ; Wetenschappelijke technieken ; Aufsatzsammlung ; Polymerase-Kettenreaktion ; Quantitative Analyse ; Gen ; Réaction en chaãine de la polymérase ; Polymerase Chain Reaction--methods--https://idnlmnihgov/mesh/D016133Q000379 ; DNA--analysis--https://idnlmnihgov/mesh/D004247Q000032 ; RNA--analysis--https://idnlmnihgov/mesh/D012313Q000032
Internet Access
Description Access URL
Table of contents
Table of contents
Library Call Number Additional Info Location Last
EKCM  QP606.D46Q36 1999 CEMM/GEMMD Library/Gulf Breeze,FL 09/28/2015
ELDM  QP606.D46Q36 1999 CCTE/GLTED Library/Duluth,MN 08/11/2000
Collation x, 304 pages : illustrations ; 24 cm.
Includes bibliographical references and index.
Contents Notes
TABLE OF CONTENTS -- Quantitative PCR : a survey of the present technology -- General principles of quantitative PCR -- Effects of collection, processing, and storage on RNA detection and quantification -- Quantitative RT-PCR -- Kinetic quantitative PCR vs end-point quantitative PCR with internal standard -- Comparison of competitive PCR and positive control-based PCR -- End-point titration-PCR and positive PCR and positive control-based PCR -- End-point titration-PCR for quantitation of cytomegalovirus DNA -- Analysis of amplified DNA moleculkes by capillary electrophoresis and laser induced fluorescence -- Competitor calibration and analysis of competitive amplified PCR products by high-performance liquid chromatography (HPLC) -- Quantifying amplicons with ELISA. TABLE OF CONTENTS CONT'D -- Competitive PCR quantitation utilizing a microtiter plate based format for the detection of PCR products -- Competitive and differential RT-PCR (CD-RT-PCR) for measurement of normalized gene expression using antisense competitors -- Amplified assay for specific dual-labeled DNA using the coagulation cascade (EDNA-ELCA) -- Quantitative PCR with internal standardization and OLA-ELISA product analysis for the p53 tumor suppressor gene -- Quantitative analysis of human DNA sequences by PCR and solid-phase minisequencing -- High resolution PCR quantitation by amplisensor assay -- Construction of polycompetitors for competitive PCR -- Tailed RT-PCR for the quantitation of Chloramphenicol acetyl transferase (CAT)mRNA -- A stochastic PCR approach for RNA quantification in multiple samples -- Quantitation of mRNA species by RT-PCR on total mRNA population with nonradioactive probes.