| Main Title |
Characterization of Normal Human Lung Lymphocytes and Interleukin-2-Induced Lung T Cell Lines. |
| Author |
Becker, S. ;
Harris, D. T. ;
Koren, H. S. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;ABB Environmental Services, Inc., Chapel Hill, NC. |
| Publisher |
c1990 |
| Year Published |
1990 |
| Report Number |
EPA/600/J-90/527; |
| Stock Number |
PB91-213504 |
| Additional Subjects |
Lung ;
T lymphocytes ;
Cell line ;
Interleukin-2 ;
Humans ;
Bronchoalveolar lavage fluid ;
Monoclonal antibodies ;
Phenotype ;
HLA-DR antigens ;
Cytotoxic T lymphocytes ;
CD antigens ;
Immunoglobulins ;
Mitogens ;
Flow cytometry ;
Fluorescent antibody techniques ;
Fluorescence ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-213504 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
Lymphocytes from the lower respiratory tract were obtained by bronchoalveolar lavage of healthy, non-smoking individuals. Various monoclonal antibodies characterizing activated T cells, helper-inducer and suppressor-inducer T cell subsets, and naive versus memory cells were used to define the phenotype of these lymphocytes. The highly variable CD4/CD8 ratio (0.3 to 6.6; mean=2.1) and the large proportion of the T cells expressing HLA-DR (9 to 38%; mean=21%) sugested that the T cells were recently activated by antigens selectively stimulating either helper or cytotoxic/suppressor T cell function. The majority (>70%) of the CD4+ helper T cells was CD45RO+ CD45RA-, a phenotype found on T cells that provide help for B cell immunoglobulin synthesis. Lung T cells proliferated poorly in response to phytohemagglutinin and concanavalin A but did respond to activation with low concentrations of anti-CD3 mAB (2 to 25 ng/ml) and to interleukin-2 (IL-2) alone to similar extent as did autologous peripheral blood lymphocytes. |
