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Main Title Ring Fission of Anthracene by a Eukaryota.
Author Hammel, K. E. ; Green, B. ; Gai, W. Z. ;
CORP Author State Univ. of New York Coll. of Environmental Science and Forestry, Syracuse. Dept. of Chemistry.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1993
Year Published 1993
Report Number EPA-R-813530; EPA/600/J-93/191;
Stock Number PB93-199503
Additional Subjects Aromatic polycyclic hydrocarbons ; Pollutants ; Biodeterioration ; Anthracene ; Lignin ; Land pollution ; Air pollution ; Sediments ; Fungi ; Biochemistry ; Oxidation ; In vivo analysis ; Carbon dioxide ; Reprints ; Ring fission ; Phanerochaete chrysosporium ; Chemical reaction mechanisms
Library Call Number Additional Info Location Last
NTIS  PB93-199503 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
Ligninolytic fungi are unique among eukaryotes in their ability to degrade polycyclic aromatic hydrocarbons (PAHs), but the mechanism for this process is unknown. Although certain PAHs are oxidized in vitro by the fungal lignin peroxidases (LiPs) that catalyze ligninolysis, it has never been shown that LiPs initiate PAH degradation in vivo. To address these problems, the metabolism of anthracene (AC) and its in vitro oxidation product, 9,10-anthraquinone (AQ), was examined by chromatographic and isotope dilution techniques in Phanerochaete chrysosporium. The fungal oxidation of AC to AQ was rapid, and both AC and AQ were significantly mineralized. Both compounds were cleaved by the fungus to give the same ring-fission metabolite, phthalic acid, and phthalate production from AQ was shown to occur only under ligninolytic culture conditions. These results show that the major pathway for AC degradation in Phanerochaete proceeds AC -> AQ -> phthalate + CO2 and that it is probably mediated by LiPs and other enzymes of ligninolytic metabolism.