Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Differential Cytotoxic Sensitivity in Mouse and Human Cell Lines Exposed to Organophosphate Insecticides.
Author Veronesi, B. ; Ehrich, M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;Virginia-Maryland Regional Coll. of Veterinary Medicine, Blacksburg, VA.
Publisher 1993
Year Published 1993
Report Number EPA/600/J-94/039;
Stock Number PB94-137155
Additional Subjects Organophosphate insecticides ; Toxicity ; Cell line ; Species diversity ; Cell survival ; Mice ; Humans ; Esterases ; Cytochrome P-450 ; Dose-response relationships ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB94-137155 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 05/14/1994
Collation 7p
Neuroblastoma cell lines were used to examine the differential interspecies response (i.e., species selectivity) to organophosphates (OPs). Baseline activities of the major target esterases, i.e., cholinesterase, carboxylesterase, and neurotoxic esterase, were assayed in mouse and several human neural candidate cell lines. These activities were found to be variable within individual cell lines and among the various tested cell lines. Cytotoxicity data using the neutral red fluorometric assay were collected on both human (SH-SY5Y) and mouse (NB41A3) neuroblastoma clones exposed to a variety of OP insecticides. IC50 data indicated that the tested mouse cell line was consistently more sensitive than the human cell line to equimolar doses of various OP compounds (e.g., mipafox, parathion, paraoxon, DFP, leptophos oxon, fenthion, and fenitrothion). These data suggest that interspecies-selectivity in response to OP-related cytotoxicity is influenced by intercellular differences in metabolism and baseline esterase activities.