Record Display for the EPA National Library Catalog


Main Title Novel Pathway of Toluene Catabolism in the Trichloroethylene-Degrading Bacterium G4.
Author Shields, M. S. ; Montgomery, S. O. ; Chapman, P. J. ; Cuskey, S. M. ; Pritchard, P. H. ;
CORP Author Technical Resources, Inc., Gulf Breeze, FL.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1989
Year Published 1989
Report Number EPA-68-03-3479; EPA/600/J-89/168;
Stock Number PB90-129537
Additional Subjects Toluene ; Biodeterioration ; Graphs(Charts) ; Chemical reactions ; Cresols ; Pyrocatechols/Methyl ; Reprints ; Biological pathways ; Catabolism ; Trichloroethylene ; Enzyme activation ; High performance liquid chromatography ; Mixed function oxidases
Library Call Number Additional Info Location Last
NTIS  PB90-129537 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
o-Cresol and 3-methylcatechol were identified as successive transitory intermediates of toluene catabolism by the trichloroethylene-degrading bacterium G4. The absence of a toluene dihydrodiol intermediate or toluene dioxygenase and toluene dihydrodiol dehydrogenase activities suggested that G4 catabolizes toluene by a unique pathway. Formation of a hybrid species of 18O- and 16O-labeled 3-methylcatechol from toluene in an atmosphere of 180(2) and 160(2) established that G4 catabolizes toluene by successive monooxygenations at the ortho and meta positions. Detection of trace amounts of 4-methylcatechol from toluene catabolism suggested that the initial hydroxylation of toluene was not exclusively at the ortho position. Further catabolism of 3-methylcatechol was found to proceed via catechol-2,3-dioxygenase and hydroxymuconic semialdehyde hydrolase activities. (Copyright (c) 1989 American Society for Microbiology.)