Record Display for the EPA National Library Catalog


Main Title Modified Agar Medium for Detecting Environmental Salmonellae by the Most-Probable-Number Method.
Author Hussong, D. ; Enkiri, N. K. ; Burge, W. D. ;
CORP Author Agricultural Research Service, Beltsville, MD. Biological Waste Management and Organic Resources Lab. ;Maryland Conservation Service, Beltsville.;Environmental Protection Agency, Cincinnati, OH. Water Engineering Research Lab.
Year Published 1984
Report Number EPA/600/J-84/313;
Stock Number PB85-225308
Additional Subjects Culture media ; Salmonella ; Bacteria ; Microorganisms ; Detection ; Cultures(Biology) ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB85-225308 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
Salmonellae in the environment remain a potential source of disease. Low numbers of salmonellae have been detected and enumerated from environmental samples by most probable number methods that require careful colony selection from plated agar medium. A modified xylose lysine brilliant green medium was prepared to control the loss of selectivity caused by heating the brilliant green component. Added agar reduced colony spreading. The medium contained 47 g of xylose lysine agar base per liter; the agar content was adjusted to 2%, autoclaved, cooled to 50 degrees C, and then amended just before pouring to include H2S indicator and 7 ppm (7 ml of 1:1,000 brilliant green per liter) of unheated brilliant green dye. H2S-positive salmonellae were easily detected from sewage sludge compost to the exclusion of most other gram-negative bacteria.