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Main Title Immunochemical protocols /
Other Authors
Author Title of a Work
Manson, Margaret M.
Publisher Humana Press,
Year Published 1992
OCLC Number 24870840
ISBN 0896032043; 9780896032040; 0896032701; 9780896032705
Subjects Immunochemistry--Methodology ; Antibodies--isolation & purification--https://idnlmnihgov/mesh/D000906Q000302 ; Immunochemistry--methods--https://idnlmnihgov/mesh/D007120Q000379 ; Immunochimie ; Techniques immunologiques ; Immunochemie ; Wetenschappelijke technieken ; Immunchemie--(DE-588)4161366-1 ; Methode--(DE-588)4038971-6 ; Immunochimie--Technique ; Aufsatzsammlung
Internet Access
Description Access URL
Publisher description
Library Call Number Additional Info Location Last
EKCM  QR183.6.I43 1992 CEMM/GEMMD Library/Gulf Breeze,FL 02/18/1994
Collation xiii, 480 pages : illustrations ; 24 cm.
Includes bibliographical references and index.
Contents Notes
Production of polyclonal antisera -- Efficient elution of purified proteins from polyvinylidene difluoride membranes (immobilon) after transfer from SDS-PAGE and their use as immunogens -- Raising polyclonal antibodies using nitrocellulose-bound antigen -- Synthesis of peptides for use as immunogens -- Production and characterization of antibodies against synthetic peptides -- Preparation and testing of monoclonal antibodies to recombinant proteins -- Screening of monoclonal antibodies using antigens labeled with acetylcholinesterase -- Purification of immunoglobulin G (IgG) -- Epitope mapping -- Enzyme-antienzyme method for immunohistochemistry -- Double label immunohistochemistry on tissue sections using alkaline phosphatase and peroxidase conjugates -- Immunohistochemical detection of bromodeoxyuriding-labeled nuclei for in vivo cell kinetic studies -- Avidin-biotin technology: preparation of biotinylated probes -- Avidin-biotin technology: preparation of avidin conjugates -- Immunochemical applications of avidin-biotin technology -- Preparation of gold probes -- Immunogold probes for light microscopy -- Immunogold probes in electron microscopy -- Electron microscopic silver enhancement for double labeling with antibodies raised in the same species -- Quantitative and qualitative immunoelectrophoresis -- Rocket immunoelectrophoresis techniques or electroimmunodiffusion -- Crossed immunoelectrophoresis -- Crossed immunoaffinoelectrophoresis -- Immunodetection of proteins by western blotting -- Erasable western blots -- Colloidal gold staining and immunoprobing on the same western blot -- Colloidal gold staining and immunodetection in 2-D protein mapping -- Fluorescent protein staining on nitrocellulose with subsequent immunodetection of antigen -- Competitive ELISA -- Twin-site ELISAs for fos and myc oncoproteins using the AMPAK system -- Preparation of cytotoxic antibody-toxin conjugates -- Immunoaffinity purification and quantification of antibody-toxin conjugates -- An immuno-slot-blot assay for detection and detection and quantitation of alkyldeoxyguanosines in DNA -- Production of monoclonal antibodies for the detection of chemically modified DNA -- Sensitive competitive enzyme-linked immunoassay for quantitation of modified bases in DNA -- An immunochemical assay for detecting transition of B-DNA to Z-DNA -- Cell sorting using immunomagnetic beads -- Cell preparation for flow cytometry -- Preparation of rat lung cells for flow cytometry -- The isolation of rat hepatocytes for flow cytometry -- Flow cytometric analysis of cells using an antibody to a surface antigen -- Multiple immunofluorescence analysis of cells using flow cytometry -- Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine -- Production and use on nonradioactive hybridization probes -- Cellular human and viral DNA detection by nonisotopic in situ hybridization -- Chromosomal mapping of genes by nonisotopic in situ hybridization -- Nonisotopic in situ hybridization -- Biotinylated probes in colony hybridization -- Screening of agt11 cDNA libraries using monoclonal antibodies -- Expression of foreign genes in mammalian cells using an antibody fusion system.