Record Display for the EPA National Library CatalogRECORD NUMBER: 22 OF 22
|Main Title||Validation of chemical and biological techniques for evaluation of vapors in ambient air/mutagenicity testing of twelve (12) vapor-phase compounds /|
|Author||Hughes, T. ; Sparacino, C. ; Frazier, S.|
|CORP Author||Research Triangle Inst., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.|
|Publisher||U.S. Environmental Protection Agency, Health Effects Research Laboratory,|
|Report Number||EPA/600/1-84/005; EPA-68-02-3170|
|Subjects||Air--Analysis ; Mutagenicity testing|
|Additional Subjects||Mutagens ; Air pollution ; Chemical analysis ; Bioassay ; Laboratory animals ; Sampling ; Chloromethanes ; Formaldehyde ; Epichlorohydrin ; Toxicity ; Ethylene oxide ; Nitrobenzenes ; Hydrogen sulfide ; Butenes ; Propylene ; Acetylene ; Ames bioassay ; Toxic substances ; Tenax resins ; Methane/chloro ; Ethylene/trichloro ; Aroclors ; Ethane/dichloro ; Propane/nitro|
|Collation||204 pages ; 28 cm|
The overall objective of this research was to further validate Ames mutagenicity preincubation system for detection of vapors in ambient air. Parametes for detection of vapors in the Ames/Salmonella mutagenicity assay were investigated and 12 vapor-phase compounds were tested for mutagenic potential in the above validated protocol with strains TA97, TA98 and TA100, both with and without Aroclor-induced hamster and rat liver S9. The 12 vapor-phase compounds tested were: methylene chloride, formaldehyde, trichloroethylene, ethylene dichloride, epichlorohydrin, nitropropane, nitrobenzene, hydrogen sulfide, butene, propylene, acetylene, and ethylene oxide. Ethylene oxide, epichlorohydrin, ethylene dichloride and methylene chloride were mutagenic.
Caption title. "March 1984." "EPA-600/1-84-005." Microfiche.