Record Display for the EPA National Library Catalog


Main Title Induction of Metal Binding Proteins in Striped Bass, 'Morone saxatilus,' Following Cadmium Treatment.
Author Baksi, S. M. ; Libbus, N. ; Frazier, J. M. ;
CORP Author Johns Hopkins Univ., Baltimore, MD. School of Hygiene and Public Health.;Environmental Research Lab., Narragansett, RI.
Publisher c1988
Year Published 1988
Report Number PHS-ES-03737 ;PHS-EC-000454; EPA/600/J-88/434 ; ERLN-P07
Stock Number PB90-125881
Additional Subjects Cadmium ; Kinetics ; Liver ; Metabolism ; Reprints ; Striped bass ; Metalloproteins ; Morone saxatilus ; Gel chromatography ; Polyacrylamide gel electrophoresis
Library Call Number Additional Info Location Last
NTIS  PB90-125881 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
The time course of induction of cytosolic metal binding proteins (MBP) was observed following up to three daily intramuscular injections of cadmium chloride (0.2 mg cadmium/injection). Low molecular weight binding proteins were resolved by gel permeation chromatography on Sephadex G-75. Based on total metal binding capacity, the concentration of crude MBP increased 2.6 fold. This level of induction of MBP was confirmed by polarographic analysis. Initial binding of cadmium to MBP resulted in displacement of zinc, while at later times, zinc associated with MBP increased above control levels. Using 35S-cysteine incorporation, it was shown that the rate of hepatic MBP synthesis was significantly greater than controls and sham injected fish 18 hr after the third cadmium injection. Due to interfering proteins of molecular weights similar to the metal binding proteins one dimensional PAGE was not capable of verifying induction. However, the metal binding proteins were resolved using two dimensional gel electrophoresis. (Copyright (c) 1988 Pergamom Press plc.)