Record Display for the EPA National Library Catalog


Main Title Persistence of Lymphocytes with Dicentric Chromosomes Following Whole-Body X Irradiation of Mice.
Author Kilgerman, A. D. ; Halperin, E. C. ; Erexson, G. L. ; Honore, G. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Duke Univ. Medical Center, Durham, NC. Dept. of Radiology. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/431;
Stock Number PB91-171868
Additional Subjects Chromosome aberrations ; X-rays ; Lymphocytes ; Mice ; Kinetics ; Statistical analysis ; Metaphase ; Leukocytes ; Whole body irradiation ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB91-171868 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Thirty-six male C57B1/6 mice were whole-body x-irradiated with 3 Gy to generate lymphocytes with dicentric chromosomes to study the persistence of these lymphocytes in the spleen and peripheral blood to estimate the lifespan of mature B- and T-cells. Blood and spleen were removed from groups of four mice immediately after radiation exposure and on days 1, 3, 7, 14, 28, 56, and 12, thereafter. The initial frequencies of dicentric chromosomes with accompanying fragments observed in splenic T-cells (0.44), splenic B-cells (0.43), and peripheral blood lymphocyte cultures (0.48) initiated on day 0 were not significantly different. For both splenic and peripheral blood-T-lymphocytes, the frequency of cells containing dicentric chromosomes declined in an exponential manner following irradiation, with a 50% reduction in frequency occurring 14 days after exposure. In contrast, the frequency of B-cells containing dicentric chromosomes remained stable through day 7 but then declined precipitously between day 7 and 14 and remained relatively stable, although slightly above baseline, through day 112 post-exposure.