Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Induction of Regulated Hypothermia in Mice by Urine Administration.
Author Gordon, C. J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/043;
Stock Number PB90-217514
Additional Subjects Hypothermia ; Urine ; Sodium chloride ; Mice ; Graphs(Charts) ; Dialysis ; Reprints ; Toxic substances ; Body temperature regulation
Library Call Number Additional Info Location Last
NTIS  PB90-217514 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 08/27/1990
Collation 7p
In the first experiment, mice were placed in a temperature gradient and were permitted to select their preferred ambient temperature (Ta). After one hour, the animals were removed from the gradient, injected with mouse urine at doses of 0, 6, 12, or 18 ml/kg (i.p.) and were placed quickly back in the gradient. The 18 ml/kg dose elicited a decrease in the selected Ta along with a significant reduction in body temperature measured at 60 and 120 min post-injection. In a second experiment it was found that urine collected from animals maintained at Ta's of 20, 30, or 35C overnight had similar effects on the behavioral thermoregulatory response. In the third experiment, mouse urine was dialyzed against saline using dialysis tubing with a molecular cutoff of 1000 daltons. The dialyzed urine had no effect on behavioral thermoregulation but did elicit a significant drop in body temperature; however, the decrease in temperature was not as great as that following administration of undialyzed urine. The studies indicate that mouse urine contains toxic substances that evoke a decrease in the set-point. Interestingly, the response to urine is similar to that following acute exposure to xenobiotic chemical substances.