Record Display for the EPA National Library Catalog


Main Title Estimating the Rate of Plasmid Transfer: An End-Point Method.
Author Simonsen, L. ; Gordon, D. M. ; Stewart, F. M. ; Levin., B. R. ;
CORP Author Massachusetts Univ., Amherst. Dept. of Zoology. ;Brown Univ., Providence, RI.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/551;
Stock Number PB92-129626
Additional Subjects Plasmids ; Escherichia coli ; Genetic conjugation ; Kinetics ; Computerized simulation ; Growth ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB92-129626 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
The document describes a method for determining the rate parameter of conjugative plasmid transfer that is based on single estimates of donor, recipient and transconjugant densities and the growth rate in exponential phase of the mating culture. The formula for estimating the plasmid transfer rate, gamma, was derived from a mathematical model describing cell growth and plasmid transfer in batch culture. Computer simulations were used to explore the sensitivity of the method to the realities of bacterial life, such as growth rate differences, plasmid segregation and transitory derepression of pilus synthesis. As predicted by the theory, mating experiments with the plasmid R1 in Escherichia coli K12 demonstrated that the estimate gamma is unaffected by cell density, donor: recipient ratio and mating time. Unlike previous techniques, the method allows investigations into the effect of environmental factors on plasmid transfer rates when these factors also influence population growth rates. To illustrate this, the authors examined the effect of temperature on the rate of plasmid transfer. (Copyright (c) 1990 SGM.)