||Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Kentucky Univ., Lexington.;Health Effects Research Lab., Research Triangle Park, NC. Carcinogenesis and Metabolism Branch.
Benzo(b)fluoranthene (B(b)F) was administered (100 mg/kg by i.p. injection) to male Sprague-Dawley rats. Lungs, livers, and peripheral blood lymphocytes (PBLs) were harvested 1, 3, 5, 7 14, 28, and 56 days after treatment. Several DNA adducts were observed in each tissue, with maximal levels occurring at about 7 days after treatment. Lung DNA exhibited consistently higher adduct levels than liver or PBL DNA. No significant differences were observed between tissues in the types of adducts produced. Sister chromatid exchanges (SCEs) from whole blood cultures were significantly increased relative to concurrent controls between 1 and 14 days after B(b)F administration, with maximum levels at 14 days.