||Spatial Distribution of Sperm-Derived Chromatin in Zygotes Determined by Fluorescence In situ Hybridization.
Brandriff, B. F. ;
Gordon, L. A. ;
||Lawrence Livermore National Lab., CA. Biomedical Sciences Div.;Environmental Protection Agency, Washington, DC. Office of Health and Environmental Assessment.;Department of Energy, Washington, DC.
||DE-W-7405-ENG-48; EPA/600/J-93/172 ; OHEA-R-475
Fluorescence in situ hybridization ;
DNA probes ;
Human pair 19 chromosomes ;
Y chromosome ;
Human pair 4 chromosomes ;
Female genetic risk
||Some EPA libraries have a fiche copy filed under the call number shown.
Fluorescence in situ hybridization was used to determine the spatial distribution of chromatin in zygote pronuclei. A hybrid system involving golden hamster eggs and individual human sperm permitted use of DNA probes specific for the entire human chromosome 4, for the heterochromatic region on the long arm of the human Y chromosome and for unique DNA sequences on human chromosome 19. Chromosome 4 occupied a circumscribed domain in the pronuclei, similar to findings in somatic interphases. Unlike the situation in somatic interphases, the Y heterochromatin was extended throughout the first cell cycle. Pronuclear chromatin was extended 3- to 4-fold compared to somatic interphase chromatin. The extended pronuclear chromatin conformation is likely to affect a zygote's susceptibility to environmental hazards. (Copyright (c) 1992 Elsevier Science Publishers B.V.)