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Main Title Metabolism of Chlorinated Methanes, Ethanes, and Ethylenes by a Mixed Bacterial Culture Growing on Methane.
Author Henson, J. M. ; Yates, M. V. ; Cochran, J. W. ;
CORP Author RMT, Inc., Greenville, SC. ;California Univ., Riverside. Dept. of Soil and Environmental Sciences. ;Illinois Dept. of Energy and Natural Resources, Champaign. Hazardous Waste Research and Information Center. ;Northrop Services, Inc., Ada, OK.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Publisher c1989
Year Published 1989
Report Number EPA-R-812220; EPA/600/J-89/489;
Stock Number PB91-144774
Additional Subjects Water pollution control ; Land pollution ; Biodeterioration ; Chlorine aliphatic compounds ; Cultures(Biology) ; Subsurface investigations ; Microorganisms ; Methane ; Waste disposal ; Soil contamination ; Ground water ; Natural gas ; Ethanes ; Ethylenes ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB91-144774 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Soil was taken from the top 10 cm of a soil column that removed halogenated aliphatic hydrocarbons in the presence of natural gas. The soil was used as an enrichment inoculum to determine that the removals seen in the soil column were in fact of a microbiological nature. Methane served as the source of carbon and energy and was consumed immediately by the enrichments. After several transfers of the enrichments, a stable consortium of at least three bacterial types was obtained. The predominant bacterium was a non-motile, gram-negative coccus. The stable consortium was able to remove chlorinated methanes, ethanes, and ethylenes when grown with methane and oxygen in the headspace. Methane was required for the removals to be observed. Acetylene inhibited th removals, which further suggests the involvement of methanotrophs. Benzene and toluene were removed by the mixed culture with or without methane in the headspace. Fatty acid analysis of the mixed culture resulted in a profile that indicated that the predominant organism was a type II methanotroph. The study provides further evidence that methanotrophic bacteria are capable of cometabolizing a wide range of chlorinated methanes, ethanes, and ethylenes. (Copyright (c) 1989 Society for Industrial Microbiology.)