Record Display for the EPA National Library Catalog


Main Title Metabolism of Benzo(a)pyrene and DNA (Deoxy-Ribonucleic Acid) Adduct Formation in Cultured Human Epidermal Keratinocytes.
Author Theall, Gail ; Eisinger, Magdalena ; Grunberger, Dezider ;
CORP Author Columbia Univ., New York. Coll. of Physicians and Surgeons.;Environmental Protection Agency, Research Triangle Park, NC.
Year Published 1981
Report Number EPA-CR-807282; EPA-600/J-81-610;
Stock Number PB83-164129
Additional Subjects Deoxyribonucleic acids ; Humans ; Epidermis ; Cells(Biology) ; Reprints ; Benzopyrene ; Keratinocytes
Library Call Number Additional Info Location Last
NTIS  PB83-164129 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Cultured human epidermal cells which require no feeder layer were used to study metabolism of benzo(a)pyrene (BP) and DNA adduct formation. The cultures were prepared from a single cell suspension and maintained at a pH of 5.9-6.2. At 2 micro M BP some cell toxicity was observed, and substantial cell death occurred at 4 micro M BP. The metabolism and DNA binding of BP were followed from 6 to 48 h of incubation. High pressure liquid chromatography (h.p.l.c.) revealed that BP was metabolized into 9,10-diol,7,8-diol, quinones, phenols and tetraols of BP. The prevalence of the minor DNA adducts varies as a function of the source of the primary skin cells. These results confirm that human cells with no feeder layer metabolize BP and the resultant DNA damage is similar to that found in other mammalian systems.