Record Display for the EPA National Library Catalog


Main Title Method 1600: Enterococci in Water by Membrane Filtration Using Membrane-Enterococcus Indoxyl-Beta-D-Glucoside Agar (mEI), July 2006.
CORP Author Environmental Protection Agency, Washington, DC. Office of Water.
Publisher Jul 2006
Year Published 2006
Report Number EPA/821/R-06/009;
Stock Number PB2007-101968
Additional Subjects Enterococci ; Water ; Membrane filters ; Filtration ; Fluid filters ; Membranes ; Procedures ; Detection ; Enumeration ; Water quality ; Fresh water ; Marine water ; Estuarine environments ; Bacteria ; Waste water ; Potable water ; Drinking water ; Shellfish ; membrane-Enterococcus Indoxyl-Beta-D-Glucoside Agar(mEI)
Library Call Number Additional Info Location Last
NTIS  PB2007-101968 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 44p
The MF method provides a direct count of bacteria in water based on the development of colonies on the surface of the membrane filter (Reference 18.5). A water sample is filtered through the membrane which retains the bacteria. Following filtration, the membrane containing the bacterial cells is placed on a selective medium, mEI agar, and incubated for 24 h at 41DGC. All colonies (regardless of color) with a blue halo are recorded as enterococci colonies. Magnification and a small fluorescent lamp are used for counting to give maximum visibility of colonies.