Record Display for the EPA National Library Catalog
RECORD NUMBER: 9 OF 11Main Title | Practical capillary electrophoresis / | ||||||||||||||||
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Author | Weinberger, Robert. | ||||||||||||||||
Publisher | Academic Press, | ||||||||||||||||
Year Published | 1993 | ||||||||||||||||
OCLC Number | 27222974 | ||||||||||||||||
ISBN | 0127423559 (acid-free paper); 9780127423555 (acid-free paper) | ||||||||||||||||
Subjects | Capillary electrophoresis ; Metodos instrumentais de analise (quimica) ; Electrophoresis--methods | ||||||||||||||||
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Collation | xv, 312 p. : ill. ; 24 cm. | ||||||||||||||||
Notes | Includes bibliographical references and index. |
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Contents Notes | Introduction -- Electrophoresis -- Microchromatographic separation methods -- Capillary electrophoresis -- Historical perspective -- Instrumentation -- Sources of information on HPCE -- Capillary electrophoresis: a family ot techniques -- Basic concepts -- Electrical conduction in fluid solution -- Language of electrophoresis -- Electroendoosmosis -- Efficiency -- Resolution -- Joule heating -- Optimizing the voltage and temperature -- Capillary diameter and buffer ionic strength -- Optimizing the capillary length -- Buffers -- Capillaries -- Capillary zone electrophoresis -- Mobility -- Buffer additives -- Solute-wall interactions -- Bandbroadening -- Applications -- Methods development -- Capillary isoelectric focusing -- Capillary coatings and electroosmotic flow -- Performing a run -- Focusing and mobilization problems -- Applications -- Size separations in capillary gels and polymer networks -- Separation mechanism -- Materials for CGE -- Applications -- Pulsed-field capillary electrophoresis -- Future of CGE -- Capillary isotachophoresis -- Separation mechanism -- Instrumental aspects -- Buffer selection. (cont) Advantages and problems with CITP -- Applications -- Electrokinetic capillary chromatography -- Micelles -- Separation mechanism -- Sources of bandbroadening -- Selecting the electrolyte system -- Elution range of MECC -- Alternative surfactant systems -- Applications and methods development -- Cyclodextrins -- Chiral recognition -- Optimization -- Capillary electrochromatography -- Electroosmotic pump -- Bandbroadening -- Applications -- Electrochromatography in perspective -- Injection -- Volumetric constraints on injection size -- Performing an injection and run -- Injection techniques -- Injection problems and solutions -- Alternative injection schemes -- Stacking -- Other enrichment techniques -- Detection -- On-capillary detection -- Detection problem -- Classes of detectors -- Limits of detection -- Bandbroadening -- Absorption detection -- Fluorescence detection -- Indirect detection -- Mass spectrometry -- Other detection techniques -- Fraction collection -- Putting it all together -- Selecting the mode of HPCE -- Requirements for robust separations. (cont) Realistic compromises -- Linear dynamic range -- Sample preparation -- Quantitative analysis -- Correction factors -- Special topics -- Field effect electroendoosmosis -- Ultrafast separations -- HPCE on a chip -- Multiple capillary systems -- Gradient and programmed systems -- Two-dimensional systems -- Immunologic trace enrichment. |