Record Display for the EPA National Library Catalog


Main Title Effects of 2,4-Dichlorophenol, a Metabolite of a Genetically Engineered Bacterium, and 2,4-Dichlorophenoxyacetate on Some Microorganism-Mediated Ecological Processes in Soil.
Author Short, K. A. ; Doyle, J. D. ; King, R. J. ; Seidler, R. J. ; Stotzky, G. ;
CORP Author Corvallis Environmental Research Lab., OR. ;NSI Technology Services Corp., Corvallis, OR. Environmental Research Lab. ;New York Univ., NY. Dept. of Biology. ;Michigan Univ., Ann Arbor. Medical School.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/144;
Stock Number PB91-219022
Additional Subjects Genetic engineering ; Pseudomonas ; Soil microbiology ; Terrestrial ecosystems ; Pesticides ; Soil contamination ; Mass fragmentography ; Metabolism ; Glucose ; Plasmids ; Fungi ; Toxicity ; Kinetics ; Reprints ; Dichlorophenols ; Dichlorophenoxyacetates
Library Call Number Additional Info Location Last
NTIS  PB91-219022 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
A genetically engineered microorganism, Pseudomonas putida PP0301 (pR0103), and the plasmidless parent strain, PP0301, were added at approximately ten to the seventh CFU/g of soil amended with 500 ppm of 2,4-dichlorophenoxyacete (2,4-D) (500 microgram/g). The degradation of 2,4-D and the accumulation of a single metabolite, identified by gas chromatography-mass spectrophotometry as 2,4-dichlorophenol (2,4-DCP), occurred only in soil inoculated with PP0301 (pR0103), wherein 2,4-DCP accumulated to >70 ppm for 5 weeks and the concentration of 2,4-D was reduced to <100 ppm. Coincident with the accumulation of 2,4-DCP was a >400-fold decline in the numbers of fungal propagules and a marked reduction in the rate of CO2 evolution, whereas 2,4-D did not depress either fungal propagules or respiration of the soil microbiota. 2,4-DCP did not appear to depress the numbers of total heterotrophic, sporeforming, or chitin-utilizing bacteria. (Copyright (c) 1991, American Society for Microbiology.)